LCMS 8040 Shimadzu

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Ultra High Sensitivity, By incorporating newly improved ion optics and collision cell technology the LCMS 8040. provides higher multiple reaction monitoring MRM sensitivity A five fold increase in sensitivity. reserpine S N ratio has been achieved by improving ion focusing and minimizing ion losses. between multi pole lenses These improvements also yield higher sensitivity for scan mode. measurements This higher sensitivity expands the potential range of LC MS MS applications. Ultra High Speed, The LCMS 8040 was designed to provide significantly higher sensitivity while maintaining the. high speed offered by the LCMS 8030 Ultrafast MRM transition speeds up to 555 MRMs per. second are achieved by Shimadzu s UFsweeper collision cell technology proprietary. high precision quadrupole machining capabilities and unique high voltage power supply. technology In addition the LCMS 8040 features the world s fastest polarity switching at. 15 msec With this high speed performance the LCMS 8040 can dramatically improve. analytical throughput,Ultra High Reliability, MRM optimization in Shimadzu s LCMS systems is based on a rapid series of automated flow. injection analyses requiring only minutes to perform Multiple compounds can be optimized. in an unattended sequence freeing the analyst from tedious work MRM parameters. optimized for the LCMS 8030 can be transferred to the LCMS 8040 making it possible to. transfer methods between systems The LCMS 8040 offers the same ease of maintenance. benefits as the LCMS 8030 and all consumables such as desolvation lines DL and ESI. capillaries are interchangeable as well,Per survey result as of May 2012. GCMS QP2010 Ultra GCMS TQ8030 LCMS 8030 LCMS 8040 LCMS 8050 LCMS 2020 LCMS IT TOF iMScope MALDI 7090. GCMS QP2010 SE,Ultra High Sensitivity with UFMS Technology.
Higher Sensitivity with Improved Ion Optics, The UF Lens is a lens system that offers both higher sensitivity and easier maintenance The optical system. UF Lens integrates two multi pole RF ion guides for higher sensitivity In addition the lens system can be removed. without tools for easy cleaning,Sprayer UF Lens, Qarray Multi Pole 1 Q1 Pre rod Q1 Post rod Q3 Pre rod. Desolvation,Line Skimmer Multi Pole 2 UFsweeper Detector. Collision Cell,Atmospheric 1st Vacuum,2nd 3rd 4 th. Pressure Chamber, RP Triple Inlets Tmp 4 Stage Differential Pumping System.
Ion losses between segments are minimized by,Previous UF Lens. utilizing quadrupole ion guides Configuration,LCMS 8030 LCMS 8040. A comparison of auto tune Q1 scan results for a standard sample a mixture. Increase in Sensitivity 8030 vs 8040, of PEG PPG and raffinose obtained from the LCMS 8030 and LCMS 8040 is. shown to the right Target ions used for ESI auto tuning are plotted m z. 65 05 168 10 256 15 344 20 652 40 1004 60 and 1224 75 A significant. sensitivity increase across a broad molecular weight range for precursor ion. transmission is demonstrated,0 500 1000 1500 2000,4 Speed Beyond Comparison Enhanced Sensitivity. Higher CID Efficiency with Improved Collision Cell. The UFsweeper II is a high sensitivity high speed collision cell that. features improved ion focusing by using high speed ion transport. technology This yields better product ion transmission in the. collision cell maintaining signal intensity and suppressing crosstalk. even for high speed or simultaneous multi component analysis The. capability for high throughput analysis is thus maintained at lower. levels of detection, Precursor Product LCMS 8030 LCMS 8040 Proportional.
Sample Name,m z m z CID efficiency CID efficiency Increase. Lidocaine 235 4 86 1 20 0 29 0 1 4,Atropine 290 1 124 1 7 2 11 0 1 5. Yohimbine 355 1 144 0 9 5 15 6 1 6,Tetracaine 265 1 176 1 15 7 32 0 2 0. Doxepin 280 1 107 1 4 4 7 2 1 6,Imipramine 281 1 86 1 16 2 22 1 1 3. Nortriptyline 264 1 233 1 3 8 7 4 1 9,Isopropylantipyrine 231 1 188 9 1 5 2 9 1 9.
Diazepam 285 0 154 0 2 4 3 9 1 6,Reserpine 609 3 195 1 3 9 6 6 1 7. The LCMS 8040 offers higher sensitivity while maintaining the measured simultaneously both the LCMS 8030 and LCMS 8040. ultrafast performance of the LCMS 8030 The figures below show accurately identified all 167 components In addition the LCMS 8040. simultaneous analysis of 167 pesticides in MRM positive negative showed improved sensitivity an average of three times higher for. ion analysis mode Even though positive and negative ions were all components. LCMS 8030 LCMS 8040,10 000 000 10 000 000,5 0 10 0 15 0 20 0 min 5 0 10 0 15 0 20 0 min. Analysis Column Shim pack FC ODS 2 0 mmI D 150 mmL 3 m Probe Voltage 4 5 kV ESI Positive mode 3 5 kV ESI Negative mode. Mobile Phase A 5 mmol L ammonium acetate Water Nebulizing Gas Flow 1 5 L min. Mobile Phase B 5 mmol L ammonium acetate Methanol Drying Gas Flow 10 L min. Gradient Program 15 B 0 min 40 B 1 3 5 min 50 B 6 min DL Temperature 250 C. 55 B 8 min 95 B 17 5 30 min 15 B 30 01 40 min Heat Block Temperature 400 C. Flow Rate 0 2 mL min,Column Temperature 40 C,Liquid Chromatograph Mass Spectrometer 5. Proven Ultra High Performance now,with Enhanced Sensitivity. Higher Sensitivity for Q1 Full Scan Spectra, The LCMS 8040 maintains the same high speed scanning UFscanning and polarity switching.
technology UFswitching utilized in the LCMS 8030, The LCMS 8040 not only maintains Shimadzu s proprietary pesticide samples Methomyl Carbaryl NAC Phoxim Benfuracarb. high speed technologies UF Technologies USP7855355 USP8188426 and Abamectin B1a is shown below The upper spectra were. which minimize sensitivity losses even at faster scan speeds it also acquired using the LCMS 8040 and the lower spectra were acquired. features improved ion optics which provide higher sensitivity for using the LCMS 8030 As shown the LCMS 8040 offers significant. MRMs and full scans A comparison of Q1 full scan spectra for sensitivity improvements for precursor ions or full scan data. Inten 10 000 000 Inten 10 000 000 Inten 10 000 000. 2 0 2 0 2 0,1 5 1 5 1 5,1 0 3 1 0 3 1 0,0 5 5 0 5 0 5. 200 300 400 500 600 m z 200 300 400 500 600 m z 200 300 400 500 600 m z. 15000 u sec 10000 u sec 5000 u sec, Inten 10 000 000 Inten 10 000 000 Inten 10 000 000. 2 0 2 0 2 0,1 5 1 5 1 5,1 0 1 0 1 0,0 5 0 5 0 5, 200 300 400 500 600 m z 200 300 400 500 600 m z 200 300 400 500 600 m z. 15000 u sec 10000 u sec 5000 u sec,Proven Interface for Robust Performance.
Robust User Friendly Interface, The LCMS 8030 and LCMS 8040 use the same interface In LCMS. analysis it is necessary to adjust interface conditions such as. temperatures and gas flow rates for optimal desolvation Spray. needle adjustment and probe position are also factors to be. Central position 2mm 3mm Integrated, evaluated In both the LCMS 8030 and LCMS 8040 temperatures. Locking screw ESI Capillary, and flows are easily controlled through the software ESI probe. position is set by a single easily accessible knob and spray needle Position. adjustment knob, protrusion is adjusted without the need for any special tools or. disassembly of the probe In addition this capillary incorporates a. Attach detach with,tapered design which reduces sample clogging.
a single flip of a lever,6 Speed Beyond Comparison Enhanced Sensitivity. Higher Sensitivity for MS MS Acquisition Modes, The increased scan sensitivity of the LCMS 8040 also applies to Mass error. scan modes specific to triple quadrupole mass spectrometers such associated with a. rise in scan speed, as product ion scanning precursor ion scanning and neutral loss. scanning Historically mass spectrometers have introduced mass. Increase in Scan Speed, deviation in linked scans such as precursor ion scans or neutral loss 300 5. scans when measured at maximum scan speeds,Precursor ion of m z 149 Dipropyl phthalate.
Inten 10 5 Inten 10 5 301 0,251 25 7 5 251 25,2 5 CH3 298 0 299 0 300 0 301 0 302 0 303 0 m z. 0 0 0 0 Typical triple quadrupole mass spectrometer. 240 0 250 0 m z 240 0 250 0 m z,Inten 10 7 Inten 10 7. 2727 u sec 6000 u sec, 8040 However Shimadzu s proprietary UFscanning technology allows. performing precursor ion scans or neutral loss scans at high speeds. 1 0 without loss of mass accuracy In addition the LCMS 8040 offers. 0 5 higher sensitivity levels Precursor scan results for eight kinds of. 0 5 phthalate esters are shown to the left Scans were performed at. two speeds 2727 u sec and 6000 u sec No mass shift is observed. 3 0 4 0 5 0 min 3 0 4 0 5 0 min at either scan speed and a significant sensitivity improvement is. Precursor Ion Scan of Standard Mixture Sample of 8 Phthalate Esters observed for the LCMS 8040. Easy Method Transfer, MRM parameters for the LCMS 8030 can be used in LCMS 8040 systems. Optimization of MRM parameters is an early step in LCMS method employing LCMS 8030 MRM conditions will be able to transfer. development Shimadzu has streamlined the MRM optimization these MRMs directly to the LCMS 8040 To demonstrate this a. process with a rapid and simplified approach based on automated method for the simultaneous analysis of 167 pesticides was. flow injection analysis Several method packages which contain transferred without modification from the LCMS 8030 to the. chromatographic and optimized MRM conditions for a variety of LCMS 8040 Increased sensitivity was obtained for all compounds. analytes including residual pesticides veterinary drugs and three example chromatograms are shown below. forensic drugs of abuse have also been released Laboratories. 10 ppb Cloquintocet mexyl 9 5X 10 ppb Pyrazosulfuron ethyl 7 4X 10 ppb Linuron 5 2X. 100 000 100 1 000, 1 50 ESI 336 20 238 00 ESI 415 20 182 10 ESI 246 90 160 00.
1 25 8040 8040 1 50 8040,0 25 8030 8030, 18 0 19 0 min 5 5 6 0 6 5 7 0 min 14 0 14 5 15 0 min. Liquid Chromatograph Mass Spectrometer 7,Ultra High Reliability to. Withstand Wide Range of Complex Matrices,Exceptional Durability. Blood plasma samples were spiked with verapamil and warfarin and warfarin by ESI was performed Chromatograms for the 1st 250th. then deproteinized according to the pretreatment process indicated and 450th measurements are shown below This resulted in 1 pg. below The area values from 450 consecutive LCMS 8040 analyses on column area repeatability of 4 18 for verapamil and 6 61. were then plotted Simultaneous analysis of verapamil by ESI and for warfarin. 3 0 Column Shim pack XR ODS II,Verapamil 2 0 mmI D 50 mmL 2 2 m. 2 5 Mobile Phase A 5 mmol L,ammonium acetate water.
2 0 Mobile Phase B Acetonitrile,Gradient Program,1 5 60 B 0 1 50 min. 90 B 1 51 3 00 min,1 0 60 B 3 01 4 50 min,Flow Rate 0 4 mL min. 0 50 100 150 200 250 300 350 400 450,200 L plasma sample. Add 200 L Acetonitrile 50 L 50 methanol aqueous solution and 50 L verapamil and warfarin standard solutions. Vortex and centrifuge 10000 rpm 3 min followed by freeze drying. Add 500 L dilution solution,Vortex and centrifuge 12000 rpm 5 min. 1 000 1 000 1 000, 6 0 1st injection 6 0 250 th injection 6 0 450 th injection.
5 0 5 0 5 0,4 0 4 0 4 0,3 0 3 0 3 0,2 0 2 0 2 0,1 0 1 0 1 0. 0 0 0 0 0 0, 0 3 0 4 0 5 0 6 0 7 min 0 3 0 4 0 5 0 6 0 7 min 0 3 0 4 0 5 0 6 0 7 min. Ultra Fast Speed Combined with Lower Femtogram Detection. Using the same chromatography parameters as described above analysis Verapamil Warfarin. of plasma samples spiked with 40 fg L verapamil and warfarin were 300 307 20 161 05. 1200 455 25 165 05, injected into the LCMS 8040 for signal to noise determination For 100. fg on column a S N ratio of 146 rms was obtained for verapamil and 250. 30 rms for warfarin, The resulting lower limits of detection for S N 3 rms were 2 05 fg for. verapamil and 9 88 fg for warfarin 100,Compounds Verapamil Warfarin 200 50.
S N 100 fg on column rms 146 30 0 0,0 25 0 50 min 0 50 0 75 1 00 min. LOD fg Calculated at S N 3 2 05 9 88, Chromatograms of 100 fg Verapamil and Warfarin On Column. 8 Speed Beyond Comparison Enhanced Sensitivity, Single Vendor Solution Provides Seamless Operation. LabSolutions LCMS Version 5, LabSolutions LCMS Version 5 is integrated workstation software used The Data Browser allows the user to analyze and compare multiple. to control LCMS 2020 8030 8040 systems Full HPLC control is sets of data such as peak detection data or LCMS spectra in the. included for LC VP and Prominence modules and for the Nexera same window It also allows LC and MS data to be linked which is. UHPLC series Standard data processing features include a especially useful when searching for or identifying compounds In. Quantitation Browser which is useful for quantitative processing of addition the popular report function was expanded to enable. multiple analytes and a Data Browser for overlaying chromatograms printing results in a wider variety of formats. Automatic MRM Optimization, Optimizing MRM parameters is an impor tant step when creating quantitative methods for triple quadrupole mass spectrometers.
LCMS 8030 8040 systems optimize MRM parameters using automated flow injection analysis an optimization function included. LabSolutions that allows anyone to optimize parameters easily. Entering precursor ions,Selecting parameters to optimize. Unknown product ions, Optimization choices include voltages for Q1 pre rod Q3. pre rod and Collision Energy CE optimization finds the. optimal value by first searching a wide range at 5 volt. This higher sensitivity expands the potential range of LC MS MS applications Ultra High Speed The LCMS 8040 was designed to provide significantly higher sensitivity while maintaining the high speed offered by the LCMS 8030 Ultrafast MRM transition speeds up to 555 MRMs per second are achieved by Shimadzu s UFsweeper collision cell technology proprietary high precision quadrupole

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