DEVELOPMENT AND VALIDATION OF ANALYTICAL METHODS FOR

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COMMUNAUT FRAN AISE DE BELGIQUE,ACAD MIE UNIVERSITAIRE WALLONIE EUROPE. UNIVERSIT DE LI GE GEMBLOUX AGRO BIO TECH,DEVELOPMENT AND VALIDATION OF ANALYTICAL. METHODS FOR QUANTIFYING ISOFLAVONES AND EQUOL IN,BIOLOGICAL MATRICES. Fr d ric DAEMS, Dissertation originale pr sent e en vue de l obtention du grade de docteur en sciences. agronomiques et ing nierie biologique,Promoteur Prof Georges Lognay.
Co promoteur Dr Jean Michel Romnee, Copyright Aux termes de la loi belge du 30 juin 1994 sur le droit d auteur et les droits. voisins seul l auteur a le droit de reproduire partiellement ou compl tement cet ouvrage. de quelque fa on et forme que ce soit ou d en autoriser la reproduction partielle ou. compl te de quelque mani re et sous quelque forme que ce soit Toute photocopie ou. reproduction sous une autre forme est donc faite en violation de la dite loi et des. modifications ult rieures, Il y a dans les chiffres une dimension qui n est pas. quantitative il est important de toujours maintenir une. attitude critique cet gard c est dire de rester conscient. des fondements et des limites de la quantification. Prof Jean Guy Pr vost 2016,Que ton alimentation soit ta premi re m decine. Hippocrate 5 me si cle av J C,Tout travail scientifique est une r alisation. communautaire et non pas l uvre d un cavalier seul Qui. sait o Darwin se serait gar en 1837 sans Gould sans. Owen et sans la vie scientifique tr s active de Londres et de. Stephen Jay Gould 1988, Daems Fr d ric 2016 D veloppement et validation de m thodes analytiques pour la.
quantification des isoflavones et de l quol dans des matrices biologiques Th se de. doctorat Universit de Li ge Gembloux Agro Bio Tech Gembloux Belgique. R sum de la th se, Les isoflavones sont des compos s naturels produits par les plantes et qui suscitent. un vaste int r t en raison de leur association avec une grande vari t d effets sur la sant. humaine Lorsqu ils sont absorb s par les humains ou les animaux la grande majorit de. ces compos s sont m tabolis s par des bact ries se trouvant dans le syst me digestif. Parmi les m tabolites microbiens obtenus l quol est probablement le compos le plus. tudi en raison de ses potentiels effets b n fiques sur notre sant Cependant tous les. humains ne sont pas capables de produire de l quol L enrichissement en quol de. certains de nos aliments comme le lait pourrait donc s av rer tre une strat gie. alternative permettant toute la population de profiter des potentiels bienfaits de ce. L objectif de cette th se consiste donc mettre en place une s rie d outils. d analyse permettant de quantifier ces compos s dans diverses matrices biologiques et. ainsi contribuer l tude de faisabilit de la production d un lait naturellement enrichi en. quol via une modification de l alimentation des vaches laiti res Pour cela quatre. proc dures analytiques recourant l utilisation d un syst me de chromatographie en. phase liquide de ultra haute performance coupl e une d tection par spectrom trie de. masse en tandem UPLC MS MS ont t d velopp es et enti rement valid es Les. m thodes mises au point ont ensuite pu tre utilis es avec succ s lors de deux tudes. exploratoires originales qui ont permis de quantifier la concentration en quol pr sente. dans les laits commercialis s en Belgique et tudier l chelle du laboratoire l volution. de la concentration en isoflavones dans des ensilages de tr fle violet Trifolium pratense. Cette th se apporte donc de nouveaux outils analytiques fiables qui permettront de. poursuivre le d veloppement des connaissances sur la possibilit de produire un lait. naturellement enrichi en quol et d tudier la m tabolisation des isoflavones chez la. vache laiti re, Isoflavones Equol UPLC MS MS Proc dure analytique Quantification. D veloppement Validation Vache Lait Ensilage Tr fle violet. Daems Fr d ric 2016 Development and validation of analytical methods for. quantifying isoflavones and equol in biological matrices PhD thesis University of. Li ge Gembloux Agro Bio Tech Gembloux Belgium,Thesis abstract. Isoflavones are natural plant substances that attract widespread interest because of. their association with a large variety of beneficial effects on human health When they are. ingested by animals or humans most of these compounds are metabolized by bacteria. present in the digestive system Among the isoflavone microbial metabolites equol is. probably the most widely studied because it seems to have numerous health benefits In. the human population however not everyone is able to produce equol Equol enrichment. of some food such as cow s milk could be an alternative strategy for making equol s. health benefits available to everyone, The main objective of the present thesis was to develop analytical tools for. quantifying these compounds in several biological matrices thus contributing to the. feasibility study being conducted on producing milk naturally enriched with equol For. this purpose four analytical procedures using ultra performance liquid chromatography. coupled with tandem mass spectrometry UPLC MS MS were developed and fully. validated The analytical tools were then applied successfully in two original exploratory. studies that sought to quantify equol concentrations in commercial cow s milk in Belgium. and study the evolution of isoflavone concentration in red clover Trifolium pratense L. silage in laboratory scale silos, This thesis describes new and reliable analytical tools that will help to improve.
current knowledge about the feasibility of producing cow s milk naturally enriched with. equol and to study isoflavone metabolization in dairy cow. Isoflavones Equol UPLC MS MS Analytical procedure Quantification. Development Validation Cow Milk Silage Red clover,Acknowledgements. Acknowledgements, Many people and institutions contributed in one way or another to this PhD thesis. all of whom are acknowledged here, My parents for my education for the values they instilled in me and for. the amazing childhood they gave me and my sister in Africa All of these. have molded me into the person that I am today I also want to thank them. for giving me the opportunity to complete the studies that enabled me to. present this work today Thank you for everything, My girlfriend Charlotte who has been by my side throughout and has. helped me to find motivation and energy to overcome problems Thank. you for your love and your patience, My promoter Prof Georges Lognay who has always believed in me and.
was there for me when I had doubts Since the beginning of my thesis he. has trusted in me and given me opportunities to pursue my ideas Through. the many discussions we had together he helped me to become more self. confident in scientific decision making, The other members of my PhD committee Dr Jean Michel Romnee Dr. ric Froidmont especially for the many papers you reviewed beyond your. remit Dr St phanie Heuskin Prof Marie Louise Scippo and Prof Yves. Beckers Thank you also Prof Jean Paul Barth l my for taking on the. role as chairman of the committee before handing over to Prof Marianne. Sindic in order to take well deserved retirement after a long career. Everyone has participated in one way or another in assessing this work and. providing precious advice over the 5 years it took to complete it. The members of the PhytoHealth project monitoring committee for the. many discussions and ideas we shared together over 5 years. My colleagues at the Walloon Agricultural Research Center past and. present and at all levels of the institution particularly those in the. Valorisation of Agricultural Products Department and the Products and. Sectors Department who contributed directly or indirectly to the. Acknowledgements, implementation of the PhytoHealth project Thank you for your help your. advice and the many good moments we had together, Christophe Jasselette from the LC laboratory in the Agricultural Product. Technology Unit for his valuable help in the development and validation. of the analytical methods the numerous analyses that he undertook over. the 5 years and the good moments we shared I would like to make special. mention of the high quality of his work, Catherine Rasse and Viviane Planchon for their availability and their. valuable help in the statistical analyses of the data. My friends who believed in me and showed interest in my research even. when they may not always have clearly understood my day to day work. The rest of my family and Charlotte s family for their support and their. continuous interest in my research, The Public Service of Wallonia Moerman funds for its financial support.
for the PhytoHealth project which gave me the opportunity to conduct this. fascinating and challenging PhD thesis in the best conditions with the best. equipment and to take part in many interesting international symposia I. would also like to thank the University of Li ge for the grant subsidies that. enabled me to give oral presentations of my work at the 83rd Acfas. congress at the Universit du Qu bec Rimouski, Finally I wish to thank all those not mentioned here for their support they. know who they are, A PhD thesis is never done alone I ll always be aware of that Thank you. everyone from the bottom of my heart,Acknowledgements. This PhD thesis was undertaken as part of the PhytoHealth project N. C2 06 VILM01 with the support of Walloon Agricultural Research Center and the. Abstract iii Daems Fr d ric 2016 Development and validation of analytical methods for quantifying isoflavones and equol in biological matrices PhD thesis University of Li ge Gembloux Agro Bio Tech Gembloux Belgium

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